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Experiment Tags: Viral Titering, Plaque Assay
Experiment Summary: After fixing and staining the infected cellular monolayer, plaques are counted so that titer viral stock samples in terms of plaque forming units (pfu) per milliliter.
Experiment Tags: Cell, Transfection, Transient transfection
Experiment Summary: Transient transfection means that the constructed plasmids are introduced into mammalian cells in some ways, but the foreign genes of the plasmid are not integrated into the genome of the mammalian cell.
Experiment Tags: T7E1, T7E1 Assay
Experiment Summary: Direct PCR product could be used for T7E1 detection.
Experiment Tags: PCR, Standard PCR
Experiment Summary: Taq polymerase is frequently used in the polymerase chain reaction (PCR), for it is able to withstand repeated heating to 95 °C without significant loss of activity, an efficient method for greatly amplifying the quantity of DNA templates.
Experiment Tags: RT-PCR, PCR
Experiment Summary: RNA is first reverse transcribed into cDNA using a reverse transcriptase as described here, the resulting cDNA is used as templates for subsequent PCR amplification using primers specific for one or more genes.
Experiment Tags: RNAi
Experiment Summary: RNAi consists of inititation and effector steps. At inititation steps, the dsRNAs get processed into 21-23 nucleotide (nt) small interfering RNAs (siRNAs) by an RNase III-like enzyme called Dicer.
Experiment Tags: Lentivirus, Recombinant Lentivirus
Experiment Summary: Transient transfection method is now widely used for generation of recombinant lentivirus. Envelope plasmid, packaging plasmid and recombinant lentivirus plasmid cotransfect 293T cells to get producing cells.
Experiment Tags: PERT Assay, Product-enhanced RT
Experiment Summary: Sensitivity was greatly increased when a PCR amplification step of the synthesized cDNA was introduced prior to product detection. These types of assays are known as product-enhanced RT (PERT) assays.
Experiment Tags: Dilution, Cell
Experiment Summary: Limiting dilution analysis attempts to determine the frequency of cells having a particular function that are present in a mixed population of cells. So limiting dilution analysis is an easy-operated method that used to measure the abundance of cells able to perform a particular function.
Experiment Tags: Lentivirus, Lentivirus Transduction
Experiment Summary: Lentivirus is a modified HIV virus that must be handled with caution although it cannot replicate in a host.
