Protocols for In vitro Transcription (IVT) and tRNA Binding Assay
Experiment Tags: T-box (T-box), EMSA (EMSA), RNA-RNA Interaction, TRNA (tRNA), In Vitro Transcription
Experiment Summary: This protocol describes the coupling of (i) “live” in vitro RNA transcription with (ii) binding by a radiolabeled, pre-formed tRNA followed by native gel electrophoresis and phosphorimager scan to visualize the complex.